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40 years within two epochs of two millennia

Kordium, V.A. (Вiopolymers and Cell, 2013)

The article-generalization deals with the main directions being developed in the Department of Cell Regulatory Mechanisms during past 40 years from the moment of the Institute of Molecular Biology and Genetics of Academy ...
Action of booster immunization with E2 CSFV on immune response elicited by marker DNA-vaccine against CSF

Pokholenko, Ia.O.; Gulko, T.P.; Deryabina, O.G.; Kordium, V.A. (Вiopolymers and Cell, 2012)

Цель. Исследовать влияние бустерной иммунизации рекомбинантным фрагментом Е2 ВКЧС на гуморальный иммунный ответ, индуцированный кандидатной маркированной ДНК-вакциной против КЧС. Методы. Наличие фрагмента гена Е2 ВКЧС ...
Amazing MSC – phenomenology, problems, solutions and opportunities

Kordium, V.A.; Irodov, D.M. (Вiopolymers and Cell, 2017)

The review briefly describes the history of prediction and discovery of mesenchymal stem cells (MSCs). The evolution of our views on the nature, functions, and status of MSCs in the organism is presented. We propose that ...
Bioaffinity sorbent based on immobilized protein A Staphylococcus aureus: development and application

Gorbatiuk, O.B.; Tsapenko, M.V.; Pavlova, M.V.; Okunev, O.V.; Kordium, V.A. (Вiopolymers and Cell, 2012)

Цель. Создание биоаффинного сорбента на основе ориентированно иммобилизованного белка А Staphylococcus aureus (SPA) через два целлюлозосвязывающих домена (СBD) Clostridium thermocellum и его применение для очистки антител. ...
Chronic diseases in the context of fundamental biology

Kordium, V.A. (Вiopolymers and Cell, 2017)

The problem of chronic diseases is analyzed from the perspective of general biology. We develop and substantiate the idea that the chronic diseases are an evolutionary mechanism of cleansing the population and the species ...
Comparison of proliferative activity of Wharton jelly mesenchymal stem cells in cultures under various gas conditions

Shuvalova, N.S.; Kordium, V.A. (Вiopolymers and Cell, 2015)

Aim. To optimize the cultivation of Wharton jelly-derived mesenchyma stem cells (WJ-MSCs) using physiological oxygen concentrations, and to compare the effect of “hypoxic” gas mixtures, based on nitrogen and argon, on their ...
Construction, expression, functional characterization and practical application of fusion protein SPA-BAPmut

Gorbatiuk, O.B.; Okunev, O.V.; Nikolaev, Yu.S.; Svyatenko, O.V.; Kordium, V.A. (Вiopolymers and Cell, 2013)

Aim. The creation of genetically engineered fusion protein SPA-BAPmut and its application as a secondary immunoreagent in immunoassays. Methods. Gene cloning, PCR, electrophoresis, DNA sequencing, bacteria cells culturing, ...
Development and characterization of porous functionalized collagen scaffolds for delivery of FGF-2

Pokholenko, Ia.O.; Chetyrkina, M.D.; Dubey, L.V.; Dubey, I.Ya.; Moshynets, O.V.; Sheludko, E.V.; Shpylova, S.P.; Degtiarova, M.I.; Kordium, V.A. (Вiopolymers and Cell, 2014)

Aim. To develop the porous functionalized collagen scaffold for the delivery of FGF-2 and studying its properties in vitro and in vivo. Methods. Porous collagen scaffolds were prepared by freeze- drying collagen I solutions ...
Development of the chromatographic medium for the affinity isolation of the recombinant hIFN-β1b based on immobilized single-chain antibodies

Pokholenko, Ia.O.; Gorbatiuk, O.B.; Okunev, O.V.; Irodov, D.M.; Degtiarova, M.I.; Palivoda, O.G.; Kordium, V.A. (Вiopolymers and Cell, 2015)

Aim. The development of a laboratory method for the production of immunoaffinity chromatography medium for the purification of the recombinant human IFN-β1b. Methods. A gene of the chimeric protein ScFvbINF-CBD was constructed ...
Distribution of transplanted human mesenchymal stem cells from Wharton’s Jelly in the central nervous systems of the EAE rats

Kovalchuk, M.V.; Deryabina, O.G.; Pichkur, L.D.; Verbovskaya, S.A.; Shuvalova, N.S.; Pichkur, O.L.; Kordium, V.A. (Вiopolymers and Cell, 2015)

Human Wharton’s Jelly MSCs (hWJ-MSCs) have a considerable advantage and potential in treating the central nervous system diseases and can be a new alternative treatment of Multiple Sclerosis (MS). Aim. To study the persistence ...
Expression and secretion of human recombinant LIF by genetically modified mammalian cells

Rymar, S.Yu.; Ruban, T.A.; Irodov, D.M.; Kordium, V.A. (Вiopolymers and Cell, 2011)

Aim. The aim of this work was to express the human LIF gene in mammalian cells and to study the secretion of recombinant LIF into culture medium. Methods. Recombinant LIF was detected by Western blot analysis and ...
Fundamental gap in fundamental biology

Kordium, V.A.; Andrienko, V.I.; Maslova, O.A.; Shuvalova, N.S.; Irodov, D.M.; Ruban, T.A.; Sukhorada, E.M.; Likhacheva, L.I.; Shpilevaya, S.P. (Вiopolymers and Cell, 2011)

The article raises the problems of intracellular spatial and temporal organization of metabolism, signaling, and energy supply of these processes. To provide cell functions, the enzymes of metabolic chains, molecules of ...
Maintenance of mesenchymal stem cells culture due to the cells with reduced attachment rate

Shuvalova, N.S.; Maslova, O.A.; Sukhorada, O.M.; Deryabina, O.G.; Kordium, V.A. (Вiopolymers and Cell, 2013)

Aim. The classic detachment techniques lead to changes in cells properties. We offer a simple method of cultivating the population of cells that avoided an influence on the surface structures. Methods. Mesenchymal stem ...
Modelling of systemic lesion of organism for development of multitarget cellular and cytokine therapy

Kordium, V.A.; Chaikovsky, Yu.B.; Irodov, D.M.; Drahulian, M.V.; Gulko, T.P.; Buchek, P.V.; Korsak, A.V.; Neverovskiy, A.V. (Вiopolymers and Cell, 2016)

In recent decades many human pathologies have taken a chronic course. Modern biology and medicine have come to conceptually new therapeutic approaches. The technologies, comprising stem cells and signaling molecules, are ...
Monitoring of transplanted human Mesenchymal Stem Cells from Wharton’s Jelly in xenogeneic systems in vivo

Kovalchuk, M.V.; Shuvalova, N.S.; Pokholenko, I.O.; Dragulyan, M.V.; Gulko, T.P.; Deryabina, O.G.; Kordium, V.A. (Вiopolymers and Cell, 2015)

Mesenchymal stem cells (MSCs) are ideal candidates for cell-based therapy aimed at tissue repair and immunomodulation. Aim. to study the survival of transplanted human MSCs from umbilical cord Wharton’s Jelly (hWJ-MSCs) ...
Monitoring of transplanted human Mesenchymal Stem Cells from Wharton’s Jelly in xenogeneic systems in vivo

Kovalchuk, M.V.; Shuvalova, N.S.; Pokholenko, I.O.; Dragulyan, M.V.; Gulko, T.P.; Deryabina, O.G.; Kordium, V.A. (Вiopolymers and Cell, 2015)

Mesenchymal stem cells (MSCs) are ideal candidates for cell-based therapy aimed at tissue repair and immunomodulation. Aim. to study the survival of transplanted human MSCs from umbilical cord Wharton’s Jelly (hWJ-MSCs) ...
Morphological characteristics of mesenchymal stem cells from Wharton jelly, cultivated under physiological oxygen tensions, in various gas mixtures

Shuvalova, N.S.; Kordium, V.A. (Вiopolymers and Cell, 2016)

Cultivation of mesenchymal stem cells (MSC) under physiological oxygen tensions has beneficial effect on their properties. Aim. To examine the effects of mild hypoxic conditions on the morphologic behavior of MSC from human ...
Proliferation of Wharton jelly mesenchymal stem cells, derived by preserving the cells with reduced attachment rate, under various gas conditions

Shuvalova, N.S.; Kordium, V.A. (Вiopolymers and Cell, 2015)

Aim. To estimate the proliferation rates in cultures of the human Wharton jelly mesenchymal stem cells (WJ-MSCs), obtained by the method of preservation of spontaneously detached cells, in various gas mixtures, containing ...
Recombinant Staphylococcal protein A with cysteine residue for preparation of affinity chromatography stationary phase and immunosensor applications

Gorbatiuk, O.B.; Bakhmachuk, A.O.; Dubey, L.V.; Usenko, M.O.; Irodov, D.M.; Okunev, O.V.; Kostenko, O.M.; Rachkov, A.E.; Kordium, V.A. (Вiopolymers and Cell, 2015)

Aim. Engineering of recombinant Staphylococcal protein A with cysteine residue (SPA-Cys) for preparation of affinity chromatography stationary phase and formation of bioselective element of immunosensor. Methods. DNA ...
Triggering effect of “therapeutic MSC”

Kordium, V.A.; Irodov, D.M.; Chaikovsky, Yu.B. (Вiopolymers and Cell, 2017)

The use of MSCs as a therapeutic agent is proven to be highly successful in many cases. However, the effects obtained are often temporary, and not leading to complete recovery. The reasons of such phenomenon are discussed ...

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